Identification of Strain-Specific Genes in Rhodococcus erythropolis Using a Modified HiCEP Method

نویسندگان

  • Yasuo Mitani
  • Tsuneaki Watanabe
  • Kazuhiro Kondo
  • Tomohiro Tamura
چکیده

The genus Rhodococcus exhibits a broad catabolic diversity that is often related to unusual genome diversity. A modified HiCEP method was applied for the identification of strain-specific genes by comparing two different strains. Rhodococcus erythropolis JCM3201 and NI86/21 were compared and 11 genes were identified as NI86/21-specific genes.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and Identification of a Sulfide/Sulfoxide Monooxygenase Gene from a Newly Isolated Rhodococcus Sp. Strain FMF

Rhodococcus FMF is a gram-positive bacterium isolated for the first time from soil samples of Tabriz refinery in Iran. This microorganism is able to catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the destruction of carbon-carbon bonds. Three structural genes, dszA, dszB, and dszC have been characterized and shown to be responsible for this phenotype. In this work,...

متن کامل

Improvement of Desulfurization Performance of Rhodococcus erythropolis IGTS8 by Assembling Spherical Mesoporous Silica Nanosorbents on the Surface of the Bacterial Cells

MCM-41 mesoporous silica is synthesized based on a self assembly method, using a quaternary ammonium template, CTAB for the adsorption of sulfur compounds from model oil (1.0 mmol/l DBT in dodecane solution). Then the adsorption capability of MCM-41 assembled on the surface of bacterium Rhodococcus erythropolis IGTS8 is examined regarding the improvement of the biodesulfurization process of...

متن کامل

Bioremediation potential of a phenol degrading bacterium, Rhodococcus erythropolis SKO-1

Biodegradation of phenol is a major focus of toxic organic compound degradation by microorganisms isolated from polluted areas. An increasing number of bacteria and fungi possessing unique biodegradation capabilities have been isolated in recent years. In this study a new isolate, Rhodococcus erythropolis SKO-1, from polluted soils in the Tehran oil refinery region, is reported. Identificati...

متن کامل

Investigation of Desulfurization Activity, Reusability, and Viability of Magnetite Coated Bacterial Cells

Background: Magnetic separation using magnetic nanoparticles can be used as a simple method to isolate desulfurizing bacteria from a biphasic oil/water system. Objectives: Magnetite nanoparticles were applied to coat the surface of Rhodococcus erythropolis IGTS8 and Rhodococcus erythropolis FMF desulfurizing bacterial cells, and the viability and...

متن کامل

The complete alk sequences of Rhodococcus erythropolis from Lake Baikal

BACKGROUND Rhodococci are bacteria able to degrade a wide range of hydrocarbons, including the alkanes present in crude oil, due to alk genes in their genomes. FINDINGS Genome sequencing of DNA from Rhodococcus erythropolis strain 4 (obtained from a deep-water bitumen mound) revealed four alk genes, and the predicted amino acid sequences coded by these genes were highly conserved, having sect...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2007